Información General
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| Nombre: | Matrícula: |
| Cerdán-Cabrera, Ana María | S17000317 |
| Correo electrónico: | CVU: |
| anamaccrisca@hotmail.com | 265682 |
| Generación: | Estatus: |
| 2017 | REGULAR |
| Línea de Generación y Aplicación del Conocimiento: | |
| LGAC 1: Biotecnología Aplicada a la Ecología y Sanidad Vegetal | |
| Proyecto de Tesis: | |
| Biological production of lignocellulosic ethanol: Pretreatment with recombinant laccases | |
Comité Asesor (Tutoral)
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Director de Tesis
Alarcón-Gutiérrez, Enrique, Dr. |
Codirector
Camas-Reyes, José Alberto, Dr. |
Asesor Interno
Sánchez-Velásquez, Lázaro Rafael, Dr. |
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Asesor Interno
Flores-Estévez, Norma, Dra. |
Asesor Externo
Ziarelli, Fabio, Dr. |
Resúmen
Lignocellulosic ethanol uses as raw material agroindustrial lignocellulosic wastes. Its production consists mainly of three stages: i) Pretreatment, lignin is degraded by physical, chemical, physico-chemical and/or biological means; ii) Hydrolysis, cellulose and/or hemicellulose are transformed to fermentable sugars by enzymes; iii) Fermentation, fermentable sugars are transformed to ethanol using yeast or bacteria.
It is possible to achieve lignin degradation (pretreatment) by enzymatic catalysis, but there is an issue because of the high cost of the enzymes required for the process and the very specific conditions needed to achieve high activity. One of the most promising enzymes for lignin degradation is laccase.
Pycnoporus sanguineus, a white rot fungus, has six laccase genes reported in GenBank databases. In this work we aim to clone and express in Pichia pastoris three of them, the ones with the highest activity reported (PslccI-1, PslccI-2, and PslccI-4) and produce the protein in liquid media. Subsequently, we will use the recombinant laccases for lignin degradation in sugarcane bagasse with the aim to strengthen fermentable sugars output and increase bioethanol production performance.
Palabras clave
Second generation bioethanol, biological pretreatment, 13C NMR, heterologous laccase.
